The brain is a high energy consuming tissue that needs a continuous supply of energy substrates from the circulation for optimal neurological function. Astrocytes are important for regulating the relationship between energy consumption and dynamic neuronal activity in the brain and are mainly involved in processes used for efficient transfer of energy substrates to neurons. Changes in astrocyte energy metabolism could therefore potentially disrupt essential neuronal processes, and this cell type has also been shown to undergo structural and biochemical changes in Alzheimer's disease (AD). Here the cell type distribution and cellular levels of the brain enzyme CKB, which is crucial for energy homeostasis and known to have decreased activity in AD, has been investigated to identify changes associated with neurodegenerative processes...Read more

The impact of different trypsins on commonly used bottom-up proteomics workflows were investigated across a range of sample types in a publication in Analytical Chemistry. Besides the type of trypsin, different protease concentrations, sample matrices, instrument acquisition techniques, and protease storage times were investigated...Read more

In a publication in Clinical Proteomics a novel orthogonal strategy based on affinity proteomics and mass spectrometry was used for analytical validation of biomarkers in longitudinally collected serum samples from Duchenne muscular dystrophy (DMD) patients...Read more

In a publication in Cancers a comprehensive mass spectrometry strategy was used to profile blood plasma samples from a large pan-cancer cohort including 15 different cancer types. Differential expression analysis and machine learning was then used to identify a potential biomarker panel able to correctly diagnose multiple myeloma...Read more

Human blood provides an easily obtainable, extensive, and sensitive diagnostic material for the assessment of wellness and disease in individuals and populations. The abundance of proteins and their primary isoforms, alternative splice isoforms, post-translational modifications (PTMs), and protein sequence variants provide a distinct snapshot into the current function of the circulatory system and all organs with which blood comes in contact...Read more