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Show complete data for human cells assay. The location(s) are highlighted in the illustration on the right.
Localized to the nucleus & cytosol.
RNA cell categoryi
The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene. The RNA-seq data was then used to classify all genes according to their cell line-specific expression into one of six different categories, defined based on the total set of all TPM values in all analyzed cell lines.
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Main locationi
The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations. It is highlighted in the illustration to the right. If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.
Localized to the Nucleus (supported), Cytosol (supported)
DATA RELIABILITY
Reliability scorei
A reliability score is set for all genes and indicates the level of reliability of the analyzed protein expression pattern based on available protein/RNA/gene characterization data. The reliability of the annotated protein expression data is also scored depending on similarity in immunostaining patterns and consistency with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database.
Below is an overview of RNA expression data generated in the HPA project. The analyzed cell lines are divided into 12 color-coded groups according to the organ they were obtained from. By clicking the toolbars in the top right corner it is possible to sort the cell lines in the chart by different criteria: the organ and the origin that the cell line was obtained from, the category of the cell line according to cellosaurus, alphabetically or by descending RNA expression. Detailed information about a specific cell line can be accessed by hovering over the corresponding bar in the chart. The RNA-sequencing results generated in the HPA are reported as number of Transcripts per Kilobase Million (TPM). In the Human Protein Atlas a TPM value of 1.0 is defined as a treshhold for expression of the corresponding protein.
The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene. The RNA-seq data was then used to classify all genes according to their cell line-specific expression into one of six different categories, defined based on the total set of all TPM values in all analyzed cell lines.
Cell lines sorted after organ of phenotypic resemblance.
Cell lines sorted after biological source for establishment.
Cell lines sorted after the cell line category according to Cellosaurus.
Cell lines sorted on descending RNA expression.
Cell lines sorted alphabetically.
HUMAN CELLSi
The "human cells" section gives an overview about the subcellular location of the protein of interest obtained by indirect immunofluorescence microscopy, an antibody-based protein-visualization technique. The immunofluorescent analysis is carried out in three different cell lines, one of them always being U-2 OS. A selection of immunofluorescent images is displayed below. Three different organelle probes are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The antibody staining targeting the protein of interest is shown in green. By using the toggle channel buttons, the different channels can be turned on and off. For the selection of the images to compare, use the checkboxes next to the images at the bottom. Three images can be compared at a time. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay. For cell structure reference, visit the cell dictionary.
Summaryi
Summary of the immunofluorescent analysis in all studied cell lines with all tested antibodies.
Localized to the nucleus & cytosol.
Main locationi
The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations.
Nucleus (supported), Cytosol (supported)
Toggle channelsi
Three different organelle probes are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The antibody staining targeting the protein of interest is shown in green. By using the "toggle channels"-buttons, the different channels can be turned on and off. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.
Low
High
G1
S
G2
M
N/A
Thumbnaili
Representative images for the assay. Three images can be compared at the same time. To change which images to compare, use the checkboxes next to the images below. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay.
Antibodyi
Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.
Cell linei
Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.
Locationi
Location(s) annotated in the corresponding cell line.
Single-cell variationi
As the images in the Cell Atlas provide single cell resolution, variations in protein expression patterns from cell to cell can be observed. A single-cell variation can either be observed in the intensity of the immunofluorescent signal or in the spatial distribution pattern of the protein. This column contains information about whether and for which of the annotated locations a single-cell variation pattern was manually annotated.
Cell cycle dependent variationi
A likely cause for single-cell variation in the immunofluorescent images is cell cycle dependency. This column contains information about whether the manually observed cell-to-cell variation pattern correlates with cell cycle progression.
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
PARK7 (HGNC Symbol)
Synonyms
DJ-1, DJ1
Description
Parkinsonism associated deglycase (HGNC Symbol)
Entrez gene summary
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
PARK7-001
PARK7-002
PARK7-003
PARK7-004
PARK7-005
PARK7-006
PARK7-010
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Q99497 [Direct mapping] Protein deglycase DJ-1 V9HWC2 [Target identity:100%; Query identity:100%] Epididymis secretory sperm binding protein Li 67p
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Enzymes ENZYME proteins Hydrolases Peptidases Cysteine-type peptidases SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Plasma proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0000785 [chromatin] GO:0001933 [negative regulation of protein phosphorylation] GO:0002866 [positive regulation of acute inflammatory response to antigenic stimulus] GO:0003713 [transcription coactivator activity] GO:0003723 [RNA binding] GO:0003729 [mRNA binding] GO:0005102 [receptor binding] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005739 [mitochondrion] GO:0005747 [mitochondrial respiratory chain complex I] GO:0005829 [cytosol] GO:0005886 [plasma membrane] GO:0005913 [cell-cell adherens junction] GO:0006469 [negative regulation of protein kinase activity] GO:0006508 [proteolysis] GO:0006517 [protein deglycosylation] GO:0006914 [autophagy] GO:0006954 [inflammatory response] GO:0007005 [mitochondrion organization] GO:0007265 [Ras protein signal transduction] GO:0007338 [single fertilization] GO:0008134 [transcription factor binding] GO:0008233 [peptidase activity] GO:0009438 [methylglyoxal metabolic process] GO:0010273 [detoxification of copper ion] GO:0010628 [positive regulation of gene expression] GO:0010629 [negative regulation of gene expression] GO:0016020 [membrane] GO:0016532 [superoxide dismutase copper chaperone activity] GO:0016605 [PML body] GO:0016684 [oxidoreductase activity, acting on peroxide as acceptor] GO:0016787 [hydrolase activity] GO:0019249 [lactate biosynthetic process] GO:0019899 [enzyme binding] GO:0019900 [kinase binding] GO:0019955 [cytokine binding] GO:0030424 [axon] GO:0031397 [negative regulation of protein ubiquitination] GO:0032091 [negative regulation of protein binding] GO:0032148 [activation of protein kinase B activity] GO:0032435 [negative regulation of proteasomal ubiquitin-dependent protein catabolic process] GO:0032757 [positive regulation of interleukin-8 production] GO:0033138 [positive regulation of peptidyl-serine phosphorylation] GO:0033234 [negative regulation of protein sumoylation] GO:0033864 [positive regulation of NAD(P)H oxidase activity] GO:0034599 [cellular response to oxidative stress] GO:0036470 [tyrosine 3-monooxygenase activator activity] GO:0036471 [cellular response to glyoxal] GO:0036478 [L-dopa decarboxylase activator activity] GO:0036524 [protein deglycase activity] GO:0036525 [protein deglycation] GO:0036526 [peptidyl-cysteine deglycation] GO:0036527 [peptidyl-arginine deglycation] GO:0036528 [peptidyl-lysine deglycation] GO:0036529 [protein deglycation, glyoxal removal] GO:0036530 [protein deglycation, methylglyoxal removal] GO:0036531 [glutathione deglycation] GO:0042743 [hydrogen peroxide metabolic process] GO:0042802 [identical protein binding] GO:0042803 [protein homodimerization activity] GO:0043066 [negative regulation of apoptotic process] GO:0043523 [regulation of neuron apoptotic process] GO:0043524 [negative regulation of neuron apoptotic process] GO:0044388 [small protein activating enzyme binding] GO:0044390 [ubiquitin-like protein conjugating enzyme binding] GO:0045121 [membrane raft] GO:0045296 [cadherin binding] GO:0045340 [mercury ion binding] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046295 [glycolate biosynthetic process] GO:0046826 [negative regulation of protein export from nucleus] GO:0048471 [perinuclear region of cytoplasm] GO:0050681 [androgen receptor binding] GO:0050727 [regulation of inflammatory response] GO:0050787 [detoxification of mercury ion] GO:0050821 [protein stabilization] GO:0051091 [positive regulation of sequence-specific DNA binding transcription factor activity] GO:0051444 [negative regulation of ubiquitin-protein transferase activity] GO:0051881 [regulation of mitochondrial membrane potential] GO:0051897 [positive regulation of protein kinase B signaling] GO:0060548 [negative regulation of cell death] GO:0060765 [regulation of androgen receptor signaling pathway] GO:0070062 [extracellular exosome] GO:0070301 [cellular response to hydrogen peroxide] GO:0070491 [repressing transcription factor binding] GO:0090073 [positive regulation of protein homodimerization activity] GO:0097110 [scaffold protein binding] GO:0098793 [presynapse] GO:0098869 [cellular oxidant detoxification] GO:1900182 [positive regulation of protein localization to nucleus] GO:1901215 [negative regulation of neuron death] GO:1901671 [positive regulation of superoxide dismutase activity] GO:1901984 [negative regulation of protein acetylation] GO:1902236 [negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway] GO:1902903 [regulation of supramolecular fiber organization] GO:1902958 [positive regulation of mitochondrial electron transport, NADH to ubiquinone] GO:1903073 [negative regulation of death-inducing signaling complex assembly] GO:1903094 [negative regulation of protein K48-linked deubiquitination] GO:1903122 [negative regulation of TRAIL-activated apoptotic signaling pathway] GO:1903135 [cupric ion binding] GO:1903136 [cuprous ion binding] GO:1903168 [positive regulation of pyrroline-5-carboxylate reductase activity] GO:1903178 [positive regulation of tyrosine 3-monooxygenase activity] GO:1903181 [positive regulation of dopamine biosynthetic process] GO:1903189 [glyoxal metabolic process] GO:1903197 [positive regulation of L-dopa biosynthetic process] GO:1903200 [positive regulation of L-dopa decarboxylase activity] GO:1903202 [negative regulation of oxidative stress-induced cell death] GO:1903206 [negative regulation of hydrogen peroxide-induced cell death] GO:1903208 [negative regulation of hydrogen peroxide-induced neuron death] GO:1903377 [negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway] GO:1903384 [negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway] GO:1903599 [positive regulation of mitophagy] GO:1905259 [negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway] GO:1990381 [ubiquitin-specific protease binding] GO:2000157 [negative regulation of ubiquitin-specific protease activity] GO:2000679 [positive regulation of transcription regulatory region DNA binding] GO:2000825 [positive regulation of androgen receptor activity] GO:2001237 [negative regulation of extrinsic apoptotic signaling pathway] GO:2001268 [negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway]
The Human Protein Atlas project is funded
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