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Show complete data for human cells assay. The location(s) are highlighted in the illustration on the right.
Localized to the nuclear speckles.
RNA cell categoryi
The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene. The RNA-seq data was then used to classify all genes according to their cell line-specific expression into one of six different categories, defined based on the total set of all TPM values in all analyzed cell lines.
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Main locationi
The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations. It is highlighted in the illustration to the right. If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.
A reliability score is set for all genes and indicates the level of reliability of the analyzed protein expression pattern based on available protein/RNA/gene characterization data. The reliability of the annotated protein expression data is also scored depending on similarity in immunostaining patterns and consistency with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database.
Below is an overview of RNA expression data generated in the HPA project. The analyzed cell lines are divided into 12 color-coded groups according to the organ they were obtained from. By clicking the toolbars in the top right corner it is possible to sort the cell lines in the chart by different criteria: the organ and the origin that the cell line was obtained from, the category of the cell line according to cellosaurus, alphabetically or by descending RNA expression. Detailed information about a specific cell line can be accessed by hovering over the corresponding bar in the chart. The RNA-sequencing results generated in the HPA are reported as number of Transcripts per Kilobase Million (TPM). In the Human Protein Atlas a TPM value of 1.0 is defined as a treshhold for expression of the corresponding protein.
The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene. The RNA-seq data was then used to classify all genes according to their cell line-specific expression into one of six different categories, defined based on the total set of all TPM values in all analyzed cell lines.
Cell lines sorted after organ of phenotypic resemblance.
Cell lines sorted after biological source for establishment.
Cell lines sorted after the cell line category according to Cellosaurus.
Cell lines sorted on descending RNA expression.
Cell lines sorted alphabetically.
HUMAN CELLSi
The "human cells" section gives an overview about the subcellular location of the protein of interest obtained by indirect immunofluorescence microscopy, an antibody-based protein-visualization technique. The immunofluorescent analysis is carried out in three different cell lines, one of them always being U-2 OS. A selection of immunofluorescent images is displayed below. Three different organelle probes are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The antibody staining targeting the protein of interest is shown in green. By using the toggle channel buttons, the different channels can be turned on and off. For the selection of the images to compare, use the checkboxes next to the images at the bottom. Three images can be compared at a time. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay. For cell structure reference, visit the cell dictionary.
Summaryi
Summary of the immunofluorescent analysis in all studied cell lines with all tested antibodies.
Localized to the nuclear speckles.
Main locationi
The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations.
Nuclear speckles (supported)
Toggle channelsi
Three different organelle probes are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The antibody staining targeting the protein of interest is shown in green. By using the "toggle channels"-buttons, the different channels can be turned on and off. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.
Low
High
G1
S
G2
M
N/A
Thumbnaili
Representative images for the assay. Three images can be compared at the same time. To change which images to compare, use the checkboxes next to the images below. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay.
Antibodyi
Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.
Cell linei
Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.
Locationi
Location(s) annotated in the corresponding cell line.
Single-cell variationi
As the images in the Cell Atlas provide single cell resolution, variations in protein expression patterns from cell to cell can be observed. A single-cell variation can either be observed in the intensity of the immunofluorescent signal or in the spatial distribution pattern of the protein. This column contains information about whether and for which of the annotated locations a single-cell variation pattern was manually annotated.
Cell cycle dependent variationi
A likely cause for single-cell variation in the immunofluorescent images is cell cycle dependency. This column contains information about whether the manually observed cell-to-cell variation pattern correlates with cell cycle progression.
For the genes where the mouse and human genes are orthologues, the mouse cell line NIH 3T3 is also stained. The main subcellular location of the encoded proteins and any additional locations are reported as well as staining characteristics, staining intensity and validation score. Example images are shown below. To change which images to compare, use the checkboxes next to the images at the bottom. Three images can be compared at a time. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay. For cell structure reference, visit the cell dictionary.
Main locationi
The main location is characterized by a higher intensity compared to other locations observed.
Three different organelle probes are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The HPA-antibody staining targeting the protein of interest is shown in green. By using the "toggle channels"-buttons, the different channels can be turned on and off. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.
Low
High
G1
S
G2
M
N/A
Thumbnaili
Representative images for the assay. Three images can be compared at the same time. To change which images to compare, use the checkboxes next to the images below. All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay.
Antibodyi
Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.
Cell linei
Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.
Locationi
Location(s) annotated in the corresponding cell line.
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
MAPK14 (HGNC Symbol)
Synonyms
CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15
Description
Mitogen-activated protein kinase 14 (HGNC Symbol)
Entrez gene summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
MAPK14-001
MAPK14-002
MAPK14-003
MAPK14-004
MAPK14-007
MAPK14-201
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Q16539 [Direct mapping] Mitogen-activated protein kinase 14 L7RSM2 [Target identity:100%; Query identity:100%] Mitogen-activated protein kinase
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Enzymes ENZYME proteins Transferases Kinases CMGC Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0000077 [DNA damage checkpoint] GO:0000166 [nucleotide binding] GO:0000187 [activation of MAPK activity] GO:0000902 [cell morphogenesis] GO:0000922 [spindle pole] GO:0001502 [cartilage condensation] GO:0001525 [angiogenesis] GO:0001890 [placenta development] GO:0002062 [chondrocyte differentiation] GO:0004672 [protein kinase activity] GO:0004674 [protein serine/threonine kinase activity] GO:0004707 [MAP kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005576 [extracellular region] GO:0005623 [cell] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005739 [mitochondrion] GO:0005829 [cytosol] GO:0006006 [glucose metabolic process] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006468 [protein phosphorylation] GO:0006915 [apoptotic process] GO:0006928 [movement of cell or subcellular component] GO:0006935 [chemotaxis] GO:0006974 [cellular response to DNA damage stimulus] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007178 [transmembrane receptor protein serine/threonine kinase signaling pathway] GO:0007265 [Ras protein signal transduction] GO:0007519 [skeletal muscle tissue development] GO:0010628 [positive regulation of gene expression] GO:0010831 [positive regulation of myotube differentiation] GO:0014835 [myoblast differentiation involved in skeletal muscle regeneration] GO:0016301 [kinase activity] GO:0016310 [phosphorylation] GO:0016607 [nuclear speck] GO:0016740 [transferase activity] GO:0018105 [peptidyl-serine phosphorylation] GO:0019395 [fatty acid oxidation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030278 [regulation of ossification] GO:0030316 [osteoclast differentiation] GO:0031281 [positive regulation of cyclase activity] GO:0031663 [lipopolysaccharide-mediated signaling pathway] GO:0032495 [response to muramyl dipeptide] GO:0032496 [response to lipopolysaccharide] GO:0034774 [secretory granule lumen] GO:0035556 [intracellular signal transduction] GO:0035924 [cellular response to vascular endothelial growth factor stimulus] GO:0035994 [response to muscle stretch] GO:0038066 [p38MAPK cascade] GO:0042307 [positive regulation of protein import into nucleus] GO:0042770 [signal transduction in response to DNA damage] GO:0043312 [neutrophil degranulation] GO:0045648 [positive regulation of erythrocyte differentiation] GO:0045663 [positive regulation of myoblast differentiation] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046326 [positive regulation of glucose import] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0051090 [regulation of sequence-specific DNA binding transcription factor activity] GO:0051146 [striated muscle cell differentiation] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051525 [NFAT protein binding] GO:0060045 [positive regulation of cardiac muscle cell proliferation] GO:0070062 [extracellular exosome] GO:0070935 [3'-UTR-mediated mRNA stabilization] GO:0071222 [cellular response to lipopolysaccharide] GO:0071479 [cellular response to ionizing radiation] GO:0090090 [negative regulation of canonical Wnt signaling pathway] GO:0090336 [positive regulation of brown fat cell differentiation] GO:0090400 [stress-induced premature senescence] GO:0098586 [cellular response to virus] GO:1900015 [regulation of cytokine production involved in inflammatory response] GO:1901741 [positive regulation of myoblast fusion] GO:1901796 [regulation of signal transduction by p53 class mediator] GO:1904813 [ficolin-1-rich granule lumen] GO:2000379 [positive regulation of reactive oxygen species metabolic process] GO:2001184 [positive regulation of interleukin-12 secretion]
Q16539 [Direct mapping] Mitogen-activated protein kinase 14 A0A024RD15 [Target identity:100%; Query identity:100%] Mitogen-activated protein kinase
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Enzymes ENZYME proteins Transferases Kinases CMGC Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0000165 [MAPK cascade] GO:0000166 [nucleotide binding] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004674 [protein serine/threonine kinase activity] GO:0004707 [MAP kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005576 [extracellular region] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006468 [protein phosphorylation] GO:0006915 [apoptotic process] GO:0006928 [movement of cell or subcellular component] GO:0006935 [chemotaxis] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007265 [Ras protein signal transduction] GO:0010628 [positive regulation of gene expression] GO:0010831 [positive regulation of myotube differentiation] GO:0016301 [kinase activity] GO:0016310 [phosphorylation] GO:0016607 [nuclear speck] GO:0016740 [transferase activity] GO:0018105 [peptidyl-serine phosphorylation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030316 [osteoclast differentiation] GO:0031281 [positive regulation of cyclase activity] GO:0034774 [secretory granule lumen] GO:0035556 [intracellular signal transduction] GO:0035924 [cellular response to vascular endothelial growth factor stimulus] GO:0038066 [p38MAPK cascade] GO:0042770 [signal transduction in response to DNA damage] GO:0043312 [neutrophil degranulation] GO:0045648 [positive regulation of erythrocyte differentiation] GO:0045663 [positive regulation of myoblast differentiation] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0051090 [regulation of sequence-specific DNA binding transcription factor activity] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051525 [NFAT protein binding] GO:0070062 [extracellular exosome] GO:0070935 [3'-UTR-mediated mRNA stabilization] GO:0071222 [cellular response to lipopolysaccharide] GO:0071479 [cellular response to ionizing radiation] GO:0090400 [stress-induced premature senescence] GO:0098586 [cellular response to virus] GO:1900015 [regulation of cytokine production involved in inflammatory response] GO:1901741 [positive regulation of myoblast fusion] GO:1901796 [regulation of signal transduction by p53 class mediator] GO:1904813 [ficolin-1-rich granule lumen] GO:2000379 [positive regulation of reactive oxygen species metabolic process] GO:2001184 [positive regulation of interleukin-12 secretion]
Q16539 [Direct mapping] Mitogen-activated protein kinase 14
Show all
Enzymes ENZYME proteins Transferases Kinases CMGC Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000166 [nucleotide binding] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004674 [protein serine/threonine kinase activity] GO:0004707 [MAP kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005576 [extracellular region] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006468 [protein phosphorylation] GO:0006915 [apoptotic process] GO:0006928 [movement of cell or subcellular component] GO:0006935 [chemotaxis] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007265 [Ras protein signal transduction] GO:0010628 [positive regulation of gene expression] GO:0010831 [positive regulation of myotube differentiation] GO:0016301 [kinase activity] GO:0016310 [phosphorylation] GO:0016607 [nuclear speck] GO:0016740 [transferase activity] GO:0018105 [peptidyl-serine phosphorylation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030316 [osteoclast differentiation] GO:0031281 [positive regulation of cyclase activity] GO:0034774 [secretory granule lumen] GO:0035556 [intracellular signal transduction] GO:0035924 [cellular response to vascular endothelial growth factor stimulus] GO:0038066 [p38MAPK cascade] GO:0042770 [signal transduction in response to DNA damage] GO:0043312 [neutrophil degranulation] GO:0045648 [positive regulation of erythrocyte differentiation] GO:0045663 [positive regulation of myoblast differentiation] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0051090 [regulation of sequence-specific DNA binding transcription factor activity] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051525 [NFAT protein binding] GO:0070062 [extracellular exosome] GO:0070935 [3'-UTR-mediated mRNA stabilization] GO:0071222 [cellular response to lipopolysaccharide] GO:0071479 [cellular response to ionizing radiation] GO:0090400 [stress-induced premature senescence] GO:0098586 [cellular response to virus] GO:1900015 [regulation of cytokine production involved in inflammatory response] GO:1901741 [positive regulation of myoblast fusion] GO:1901796 [regulation of signal transduction by p53 class mediator] GO:1904813 [ficolin-1-rich granule lumen] GO:2000379 [positive regulation of reactive oxygen species metabolic process] GO:2001184 [positive regulation of interleukin-12 secretion]
The Human Protein Atlas project is funded
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