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RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.
TCGA (cancer tissue):
Expressed in all
HPA (cell line):
Expressed in all
HPA (normal tissue):
Expressed in all
Protein evidencei
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expression normal tissuei
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining consistent with RNA expression data.
Reliability score - normal tissuesi
Reliability score (score description), divided into Enhanced, Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between antibody staining pattern, available RNA-Seq and gene/protein characterization data, as well as similarity between independent antibodies targeting the same protein.
Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.
RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).
TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name. The cancer types are color-coded according to which type of normal organ the cancer originates from.
Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.
For each cancer, color-coded bars indicate the percentage of patients (maximum 12 patients) with high and medium protein expression level. The cancer types are color-coded according to which type of normal organ the cancer originates from. Low or not detected protein expression results in a white bar. Mouse-over function shows details about expression level and normal tissue of origin. The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies.
Strong nuclear immunoreactivity was found in tumor cells.
Malignant cells displayed moderate to strong nuclear immunoreactivity.
GENE INFORMATIONi
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
HSF1 (HGNC Symbol)
Synonyms
HSTF1
Description
Heat shock transcription factor 1 (HGNC Symbol)
Entrez gene summary
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
HSF1-001
HSF1-004
HSF1-006
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Q00613 [Direct mapping] Heat shock factor protein 1
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Predicted intracellular proteins Plasma proteins Transcription factors Helix-turn-helix domains Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0000122 [negative regulation of transcription from RNA polymerase II promoter] GO:0000165 [MAPK cascade] GO:0000775 [chromosome, centromeric region] GO:0000776 [kinetochore] GO:0000777 [condensed chromosome kinetochore] GO:0000791 [euchromatin] GO:0000792 [heterochromatin] GO:0000922 [spindle pole] GO:0000978 [RNA polymerase II core promoter proximal region sequence-specific DNA binding] GO:0000979 [RNA polymerase II core promoter sequence-specific DNA binding] GO:0001078 [transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding] GO:0001162 [RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding] GO:0001701 [in utero embryonic development] GO:0001892 [embryonic placenta development] GO:0003677 [DNA binding] GO:0003682 [chromatin binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005694 [chromosome] GO:0005737 [cytoplasm] GO:0005813 [centrosome] GO:0005815 [microtubule organizing center] GO:0005829 [cytosol] GO:0005856 [cytoskeleton] GO:0006281 [DNA repair] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0006397 [mRNA processing] GO:0006468 [protein phosphorylation] GO:0006810 [transport] GO:0006952 [defense response] GO:0006974 [cellular response to DNA damage stimulus] GO:0007143 [female meiotic division] GO:0007283 [spermatogenesis] GO:0007584 [response to nutrient] GO:0008284 [positive regulation of cell proliferation] GO:0008285 [negative regulation of cell proliferation] GO:0009299 [mRNA transcription] GO:0009408 [response to heat] GO:0010243 [response to organonitrogen compound] GO:0010628 [positive regulation of gene expression] GO:0010629 [negative regulation of gene expression] GO:0010667 [negative regulation of cardiac muscle cell apoptotic process] GO:0014070 [response to organic cyclic compound] GO:0014823 [response to activity] GO:0016605 [PML body] GO:0019901 [protein kinase binding] GO:0031072 [heat shock protein binding] GO:0031490 [chromatin DNA binding] GO:0032355 [response to estradiol] GO:0032496 [response to lipopolysaccharide] GO:0032720 [negative regulation of tumor necrosis factor production] GO:0033574 [response to testosterone] GO:0034605 [cellular response to heat] GO:0034620 [cellular response to unfolded protein] GO:0035690 [cellular response to drug] GO:0035865 [cellular response to potassium ion] GO:0040018 [positive regulation of multicellular organism growth] GO:0042517 [positive regulation of tyrosine phosphorylation of Stat3 protein] GO:0042802 [identical protein binding] GO:0043200 [response to amino acid] GO:0043234 [protein complex] GO:0043280 [positive regulation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0043497 [regulation of protein heterodimerization activity] GO:0043565 [sequence-specific DNA binding] GO:0043621 [protein self-association] GO:0043623 [cellular protein complex assembly] GO:0045120 [pronucleus] GO:0045931 [positive regulation of mitotic cell cycle] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046982 [protein heterodimerization activity] GO:0048471 [perinuclear region of cytoplasm] GO:0051028 [mRNA transport] GO:0051260 [protein homooligomerization] GO:0051879 [Hsp90 protein binding] GO:0060136 [embryonic process involved in female pregnancy] GO:0061408 [positive regulation of transcription from RNA polymerase II promoter in response to heat stress] GO:0061770 [translation elongation factor binding] GO:0070207 [protein homotrimerization] GO:0070301 [cellular response to hydrogen peroxide] GO:0071222 [cellular response to lipopolysaccharide] GO:0071230 [cellular response to amino acid stimulus] GO:0071276 [cellular response to cadmium ion] GO:0071280 [cellular response to copper ion] GO:0071392 [cellular response to estradiol stimulus] GO:0071407 [cellular response to organic cyclic compound] GO:0071478 [cellular response to radiation] GO:0071480 [cellular response to gamma radiation] GO:0072738 [cellular response to diamide] GO:0090084 [negative regulation of inclusion body assembly] GO:0090261 [positive regulation of inclusion body assembly] GO:0097165 [nuclear stress granule] GO:0097431 [mitotic spindle pole] GO:0097677 [STAT family protein binding] GO:0098847 [sequence-specific single stranded DNA binding] GO:1900034 [regulation of cellular response to heat] GO:1900365 [positive regulation of mRNA polyadenylation] GO:1901215 [negative regulation of neuron death] GO:1901652 [response to peptide] GO:1902512 [positive regulation of apoptotic DNA fragmentation] GO:1903936 [cellular response to sodium arsenite] GO:1904385 [cellular response to angiotensin] GO:1904528 [positive regulation of microtubule binding] GO:1904843 [cellular response to nitroglycerin] GO:1904845 [cellular response to L-glutamine] GO:1990841 [promoter-specific chromatin binding] GO:1990904 [ribonucleoprotein complex] GO:1990910 [response to hypobaric hypoxia] GO:1990911 [response to psychosocial stress] GO:2001033 [negative regulation of double-strand break repair via nonhomologous end joining]