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RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.
TCGA (cancer tissue):
Expressed in all
HPA (cell line):
Cell line enhanced (BEWO, HUVEC TERT2, TIME)
HPA (normal tissue):
Tissue enriched (spleen)
Protein evidencei
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expression normal tissuei
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Cytoplasmic expression in endothelial cells, most abundant in spleen
IMMUNOHISTOCHEMISTRY DATA RELIABILITY
Data reliability descriptioni
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining mainly consistent with RNA expression data. Antibody staining in cells/structures not annotated, view images.
Reliability score - normal tissuesi
Reliability score (score description), divided into Enhanced, Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between antibody staining pattern, available RNA-Seq and gene/protein characterization data, as well as similarity between independent antibodies targeting the same protein.
Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.
RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).
TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name. The cancer types are color-coded according to which type of normal organ the cancer originates from.
Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.
For each cancer, color-coded bars indicate the percentage of patients (maximum 12 patients) with high and medium protein expression level. The cancer types are color-coded according to which type of normal organ the cancer originates from. Low or not detected protein expression results in a white bar. Mouse-over function shows details about expression level and normal tissue of origin. The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies.
Malignant tissues were in general negative. Distinct staining was observed in endothelial cells of tumor stroma.
GENE INFORMATIONi
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
NOS3 (HGNC Symbol)
Synonyms
ECNOS, eNOS
Description
Nitric oxide synthase 3 (HGNC Symbol)
Entrez gene summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
NOS3-001
NOS3-002
NOS3-004
NOS3-005
NOS3-006
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Enzymes ENZYME proteins Oxidoreductases SCAMPI predicted membrane proteins SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes FDA approved drug targets Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0001525 [angiogenesis] GO:0001542 [ovulation from ovarian follicle] GO:0001701 [in utero embryonic development] GO:0001974 [blood vessel remodeling] GO:0002028 [regulation of sodium ion transport] GO:0003057 [regulation of the force of heart contraction by chemical signal] GO:0003100 [regulation of systemic arterial blood pressure by endothelin] GO:0003785 [actin monomer binding] GO:0003958 [NADPH-hemoprotein reductase activity] GO:0004517 [nitric-oxide synthase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005516 [calmodulin binding] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005794 [Golgi apparatus] GO:0005829 [cytosol] GO:0005856 [cytoskeleton] GO:0005886 [plasma membrane] GO:0005901 [caveola] GO:0006527 [arginine catabolic process] GO:0006809 [nitric oxide biosynthetic process] GO:0007005 [mitochondrion organization] GO:0007263 [nitric oxide mediated signal transduction] GO:0008217 [regulation of blood pressure] GO:0008285 [negative regulation of cell proliferation] GO:0009408 [response to heat] GO:0010181 [FMN binding] GO:0010544 [negative regulation of platelet activation] GO:0014740 [negative regulation of muscle hyperplasia] GO:0014806 [smooth muscle hyperplasia] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0019430 [removal of superoxide radicals] GO:0020037 [heme binding] GO:0030324 [lung development] GO:0030666 [endocytic vesicle membrane] GO:0031284 [positive regulation of guanylate cyclase activity] GO:0031663 [lipopolysaccharide-mediated signaling pathway] GO:0034405 [response to fluid shear stress] GO:0034617 [tetrahydrobiopterin binding] GO:0034618 [arginine binding] GO:0042311 [vasodilation] GO:0043267 [negative regulation of potassium ion transport] GO:0043542 [endothelial cell migration] GO:0045454 [cell redox homeostasis] GO:0045766 [positive regulation of angiogenesis] GO:0045776 [negative regulation of blood pressure] GO:0045909 [positive regulation of vasodilation] GO:0046870 [cadmium ion binding] GO:0046872 [metal ion binding] GO:0050660 [flavin adenine dinucleotide binding] GO:0050661 [NADP binding] GO:0050880 [regulation of blood vessel size] GO:0050999 [regulation of nitric-oxide synthase activity] GO:0051346 [negative regulation of hydrolase activity] GO:0051926 [negative regulation of calcium ion transport] GO:0055114 [oxidation-reduction process] GO:0097110 [scaffold protein binding] GO:1902042 [negative regulation of extrinsic apoptotic signaling pathway via death domain receptors]
Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes FDA approved drug targets Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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