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RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue enriched, group enriched, tissue enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for data imported from The Genotype-Tissue Expression project (GTEX), FANTOM5 Consortium (FANTOM5) and internally generated Human Protein Atlas (HPA) data.
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expressioni
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Selective cytoplasmic expression in several tissues, most abundant in lymphoid tissues.
IMMUNOHISTOCHEMISTRY DATA RELIABILITY
Data reliability descriptioni
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining mainly consistent with RNA expression data.
Reliability scorei
Reliability score (score description), divided into Enhanced, Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between antibody staining pattern, available RNA-Seq and gene/protein characterization data, as well as similarity between independent antibodies targeting the same protein.
Below is an overview of RNA and protein expression data generated in the Human Protein Atlas project. Analyzed tissues are divided into color-coded groups according to which functional features they have in common. For each group, a list of included tissues is accessed by clicking on group name, group symbol, RNA bar, or protein bar. Subsequent selection of a particular tissue in this list links to the image data page.
Images of selected tissues give a visual summary of the protein expression profile furthest to the right.
The gray human body provides links to a histology dictionary when clicking on any part of the figure.
RNA expression (TPM)i
RNA-seq results generated in HPA are reported as number of transcripts per million (TPM). Each bar represents the highest expression score found in a particular group of tissues. The assay is described more in detail in Assays & Annotation.
Protein expression (score)i
Each bar represents the highest expression score found in a particular group of tissues. Protein expression scores are based on a best estimate of the "true" protein expression from a knowledge-based annotation, described more in detail under Assays & annotation. For genes where more than one antibody has been used, a collective score is set displaying the estimated true protein expression.
Protein expression data is shown for each of the 44 tissues. Color-coding is based on tissue groups, each consisting of tissues with functional features in common. Mouse-over function shows protein score for analyzed cell types in a selected tissue. To access image data click on tissue name or bar. Annotation of protein expression is described in detail in Assays & annotation.
For genes with available protein data for which a knowledge-based annotation gave inconclusive results, no protein expression data is displayed in the protein expression data overview. However, all immunohistochemical images are still available and the annotation data can be found under Primary data.
Organ
Expression
Alphabetical
RNA EXPRESSION OVERVIEWi
RNA expression overview shows RNA-data from three different sources: Internally generated Human Protein Atlas (HPA) RNA-seq data, RNA-seq data from the Genotype-Tissue Expression (GTEx) project and CAGE data from FANTOM5 project. Color-coding is based on tissue groups, each consisting of tissues with functional features in common. To access sample data, click on tissue name or bar.
HPA dataseti
HPA dataset RNA-seq tissue data is reported as mean TPM (transcripts per million), corresponding to mean values of the different individual samples from each tissue. Color-coding is based on tissue groups, each consisting of tissues with functional features in common. To access sample data, click on tissue name or bar. The RNA-seq assay is described in detail in Assays & Annotation.
RNA tissue category HPA HPA RNA tissue category (category description) is calculated based on mRNA expression levels across all tissues and include: tissue enriched, group enriched, tissue enhanced, expressed in all, mixed and not detected.
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
GTEx dataseti
GTEx dataset RNA-seq data is reported as median RPKM (reads per kilobase per million mapped reads), generated by the Genotype-Tissue Expression (GTEx) project. More information can be found in Assays & Annotation.
RNA tissue category GTEx GTEx RNA tissue category (category description) is calculated based on mRNA expression levels across all tissues and include: tissue enriched, group enriched, tissue enhanced, expressed in all, mixed and not detected.
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
FANTOM5 dataseti
FANTOM5 dataset Tissue data obtained through Cap Analysis of Gene Expression (CAGE) are reported as Tags Per Million, generated by the FANTOM5 project. More information can be found in Assays & Annotation.
RNA tissue category FANTOM5 FANTOM5 RNA tissue category (category description) is calculated based on gene expression levels across all tissues and include: tissue enriched, group enriched, tissue enhanced, expressed in all, mixed and not detected.
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
GENE INFORMATIONi
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
CASP3 (HGNC Symbol)
Synonyms
apopain, CPP32, CPP32B, Yama
Description
Caspase 3 (HGNC Symbol)
Entrez gene summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
CASP3-001
CASP3-002
CASP3-003
CASP3-004
CASP3-005
CASP3-006
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Enzymes ENZYME proteins Hydrolases Peptidases Cysteine-type peptidases Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0001666 [response to hypoxia] GO:0001782 [B cell homeostasis] GO:0002020 [protease binding] GO:0004190 [aspartic-type endopeptidase activity] GO:0004197 [cysteine-type endopeptidase activity] GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity] GO:0005123 [death receptor binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0005886 [plasma membrane] GO:0006309 [apoptotic DNA fragmentation] GO:0006508 [proteolysis] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0006974 [cellular response to DNA damage stimulus] GO:0007507 [heart development] GO:0007605 [sensory perception of sound] GO:0007611 [learning or memory] GO:0008233 [peptidase activity] GO:0008234 [cysteine-type peptidase activity] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0009411 [response to UV] GO:0009611 [response to wounding] GO:0009749 [response to glucose] GO:0010033 [response to organic substance] GO:0010038 [response to metal ion] GO:0010165 [response to X-ray] GO:0014070 [response to organic cyclic compound] GO:0016005 [phospholipase A2 activator activity] GO:0016241 [regulation of macroautophagy] GO:0016485 [protein processing] GO:0016787 [hydrolase activity] GO:0021766 [hippocampus development] GO:0030182 [neuron differentiation] GO:0030216 [keratinocyte differentiation] GO:0030218 [erythrocyte differentiation] GO:0030220 [platelet formation] GO:0030889 [negative regulation of B cell proliferation] GO:0031264 [death-inducing signaling complex] GO:0032025 [response to cobalt ion] GO:0032355 [response to estradiol] GO:0032403 [protein complex binding] GO:0032496 [response to lipopolysaccharide] GO:0034349 [glial cell apoptotic process] GO:0034612 [response to tumor necrosis factor] GO:0035094 [response to nicotine] GO:0035329 [hippo signaling] GO:0035556 [intracellular signal transduction] GO:0042060 [wound healing] GO:0042493 [response to drug] GO:0042542 [response to hydrogen peroxide] GO:0043029 [T cell homeostasis] GO:0043065 [positive regulation of apoptotic process] GO:0043066 [negative regulation of apoptotic process] GO:0043200 [response to amino acid] GO:0043525 [positive regulation of neuron apoptotic process] GO:0045121 [membrane raft] GO:0045165 [cell fate commitment] GO:0045736 [negative regulation of cyclin-dependent protein serine/threonine kinase activity] GO:0045786 [negative regulation of cell cycle] GO:0046007 [negative regulation of activated T cell proliferation] GO:0046677 [response to antibiotic] GO:0048011 [neurotrophin TRK receptor signaling pathway] GO:0051384 [response to glucocorticoid] GO:0051402 [neuron apoptotic process] GO:0071310 [cellular response to organic substance] GO:0071407 [cellular response to organic cyclic compound] GO:0097153 [cysteine-type endopeptidase activity involved in apoptotic process] GO:0097190 [apoptotic signaling pathway] GO:0097192 [extrinsic apoptotic signaling pathway in absence of ligand] GO:0097194 [execution phase of apoptosis] GO:0097200 [cysteine-type endopeptidase activity involved in execution phase of apoptosis]
Enzymes ENZYME proteins Hydrolases Peptidases Cysteine-type peptidases Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004190 [aspartic-type endopeptidase activity] GO:0004197 [cysteine-type endopeptidase activity] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0005886 [plasma membrane] GO:0006309 [apoptotic DNA fragmentation] GO:0006508 [proteolysis] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0008233 [peptidase activity] GO:0008234 [cysteine-type peptidase activity] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0016241 [regulation of macroautophagy] GO:0016787 [hydrolase activity] GO:0030182 [neuron differentiation] GO:0030216 [keratinocyte differentiation] GO:0030218 [erythrocyte differentiation] GO:0030220 [platelet formation] GO:0034612 [response to tumor necrosis factor] GO:0035329 [hippo signaling] GO:0043066 [negative regulation of apoptotic process] GO:0048011 [neurotrophin TRK receptor signaling pathway] GO:0097153 [cysteine-type endopeptidase activity involved in apoptotic process] GO:0097190 [apoptotic signaling pathway] GO:0097192 [extrinsic apoptotic signaling pathway in absence of ligand] GO:0097194 [execution phase of apoptosis] GO:0097200 [cysteine-type endopeptidase activity involved in execution phase of apoptosis]
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