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RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.
TCGA (cancer tissue):
Expressed in all
HPA (cell line):
Expressed in all
HPA (normal tissue):
Expressed in all
Protein evidencei
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expression normal tissuei
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Cytoplasmic and membranous expression in most tissues.
IMMUNOHISTOCHEMISTRY DATA RELIABILITY
Data reliability descriptioni
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining mainly not consistent with RNA expression data.
Reliability score - normal tissuesi
Reliability score (score description), divided into Enhanced, Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between antibody staining pattern, available RNA-Seq and gene/protein characterization data, as well as similarity between independent antibodies targeting the same protein.
Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.
RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).
TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name. The cancer types are color-coded according to which type of normal organ the cancer originates from.
Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.
For each cancer, color-coded bars indicate the percentage of patients (maximum 12 patients) with high and medium protein expression level. The cancer types are color-coded according to which type of normal organ the cancer originates from. Low or not detected protein expression results in a white bar. Mouse-over function shows details about expression level and normal tissue of origin. The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies.
Most cancer tissues showed cytoplasmic and membranous positivity of varying intensity. Lymphomas and gliomas were negative.
Most cancer tissues displayed moderate cytoplasmic immunoreactivity. Strongest staining was observed in lymphomas, which showed additional nuclear positivity in some cases.
GENE INFORMATIONi
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
CDC42 (HGNC Symbol)
Synonyms
CDC42Hs, G25K
Description
Cell division cycle 42 (HGNC Symbol)
Entrez gene summary
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).
The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
CDC42-001
CDC42-002
CDC42-004
CDC42-201
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Predicted intracellular proteins Plasma proteins RAS pathway related proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
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GO:0000139 [Golgi membrane] GO:0000166 [nucleotide binding] GO:0003924 [GTPase activity] GO:0005515 [protein binding] GO:0005525 [GTP binding] GO:0005737 [cytoplasm] GO:0005789 [endoplasmic reticulum membrane] GO:0005815 [microtubule organizing center] GO:0005819 [spindle] GO:0005829 [cytosol] GO:0005856 [cytoskeleton] GO:0005886 [plasma membrane] GO:0005925 [focal adhesion] GO:0007030 [Golgi organization] GO:0007163 [establishment or maintenance of cell polarity] GO:0007264 [small GTPase mediated signal transduction] GO:0007399 [nervous system development] GO:0007596 [blood coagulation] GO:0016020 [membrane] GO:0016567 [protein ubiquitination] GO:0019901 [protein kinase binding] GO:0021762 [substantia nigra development] GO:0030036 [actin cytoskeleton organization] GO:0030154 [cell differentiation] GO:0030175 [filopodium] GO:0030225 [macrophage differentiation] GO:0030307 [positive regulation of cell growth] GO:0030496 [midbody] GO:0031274 [positive regulation of pseudopodium assembly] GO:0031295 [T cell costimulation] GO:0031333 [negative regulation of protein complex assembly] GO:0031996 [thioesterase binding] GO:0032467 [positive regulation of cytokinesis] GO:0034191 [apolipoprotein A-I receptor binding] GO:0036464 [cytoplasmic ribonucleoprotein granule] GO:0038096 [Fc-gamma receptor signaling pathway involved in phagocytosis] GO:0039694 [viral RNA genome replication] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042802 [identical protein binding] GO:0043005 [neuron projection] GO:0043025 [neuronal cell body] GO:0043197 [dendritic spine] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048013 [ephrin receptor signaling pathway] GO:0051056 [regulation of small GTPase mediated signal transduction] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051233 [spindle midzone] GO:0051301 [cell division] GO:0051489 [regulation of filopodium assembly] GO:0051491 [positive regulation of filopodium assembly] GO:0051683 [establishment of Golgi localization] GO:0051988 [regulation of attachment of spindle microtubules to kinetochore] GO:0060071 [Wnt signaling pathway, planar cell polarity pathway] GO:0060997 [dendritic spine morphogenesis] GO:0061630 [ubiquitin protein ligase activity] GO:0070062 [extracellular exosome] GO:0072384 [organelle transport along microtubule] GO:0072686 [mitotic spindle] GO:1900026 [positive regulation of substrate adhesion-dependent cell spreading]
Predicted intracellular proteins Plasma proteins RAS pathway related proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0000166 [nucleotide binding] GO:0003924 [GTPase activity] GO:0005515 [protein binding] GO:0005525 [GTP binding] GO:0005622 [intracellular] GO:0005737 [cytoplasm] GO:0005789 [endoplasmic reticulum membrane] GO:0005815 [microtubule organizing center] GO:0005819 [spindle] GO:0005829 [cytosol] GO:0005856 [cytoskeleton] GO:0005886 [plasma membrane] GO:0005925 [focal adhesion] GO:0007030 [Golgi organization] GO:0007163 [establishment or maintenance of cell polarity] GO:0007264 [small GTPase mediated signal transduction] GO:0007399 [nervous system development] GO:0007596 [blood coagulation] GO:0016020 [membrane] GO:0016567 [protein ubiquitination] GO:0019901 [protein kinase binding] GO:0021762 [substantia nigra development] GO:0030036 [actin cytoskeleton organization] GO:0030154 [cell differentiation] GO:0030175 [filopodium] GO:0030225 [macrophage differentiation] GO:0030307 [positive regulation of cell growth] GO:0030496 [midbody] GO:0031274 [positive regulation of pseudopodium assembly] GO:0031295 [T cell costimulation] GO:0031333 [negative regulation of protein complex assembly] GO:0031996 [thioesterase binding] GO:0032467 [positive regulation of cytokinesis] GO:0034191 [apolipoprotein A-I receptor binding] GO:0036464 [cytoplasmic ribonucleoprotein granule] GO:0038096 [Fc-gamma receptor signaling pathway involved in phagocytosis] GO:0039694 [viral RNA genome replication] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042802 [identical protein binding] GO:0043005 [neuron projection] GO:0043025 [neuronal cell body] GO:0043197 [dendritic spine] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048013 [ephrin receptor signaling pathway] GO:0051056 [regulation of small GTPase mediated signal transduction] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051233 [spindle midzone] GO:0051301 [cell division] GO:0051489 [regulation of filopodium assembly] GO:0051491 [positive regulation of filopodium assembly] GO:0051683 [establishment of Golgi localization] GO:0051988 [regulation of attachment of spindle microtubules to kinetochore] GO:0060071 [Wnt signaling pathway, planar cell polarity pathway] GO:0060997 [dendritic spine morphogenesis] GO:0061630 [ubiquitin protein ligase activity] GO:0070062 [extracellular exosome] GO:0072384 [organelle transport along microtubule] GO:0072686 [mitotic spindle] GO:1900026 [positive regulation of substrate adhesion-dependent cell spreading]
Predicted intracellular proteins Plasma proteins RAS pathway related proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0000166 [nucleotide binding] GO:0000322 [storage vacuole] GO:0001934 [positive regulation of protein phosphorylation] GO:0002040 [sprouting angiogenesis] GO:0003161 [cardiac conduction system development] GO:0003334 [keratinocyte development] GO:0003924 [GTPase activity] GO:0005515 [protein binding] GO:0005525 [GTP binding] GO:0005737 [cytoplasm] GO:0005789 [endoplasmic reticulum membrane] GO:0005815 [microtubule organizing center] GO:0005819 [spindle] GO:0005829 [cytosol] GO:0005856 [cytoskeleton] GO:0005886 [plasma membrane] GO:0005911 [cell-cell junction] GO:0005925 [focal adhesion] GO:0006897 [endocytosis] GO:0007015 [actin filament organization] GO:0007030 [Golgi organization] GO:0007088 [regulation of mitotic nuclear division] GO:0007097 [nuclear migration] GO:0007163 [establishment or maintenance of cell polarity] GO:0007264 [small GTPase mediated signal transduction] GO:0007399 [nervous system development] GO:0007596 [blood coagulation] GO:0010628 [positive regulation of gene expression] GO:0010629 [negative regulation of gene expression] GO:0016020 [membrane] GO:0016337 [single organismal cell-cell adhesion] GO:0016567 [protein ubiquitination] GO:0019901 [protein kinase binding] GO:0021762 [substantia nigra development] GO:0030036 [actin cytoskeleton organization] GO:0030141 [secretory granule] GO:0030154 [cell differentiation] GO:0030175 [filopodium] GO:0030225 [macrophage differentiation] GO:0030307 [positive regulation of cell growth] GO:0030496 [midbody] GO:0030742 [GTP-dependent protein binding] GO:0031069 [hair follicle morphogenesis] GO:0031256 [leading edge membrane] GO:0031274 [positive regulation of pseudopodium assembly] GO:0031295 [T cell costimulation] GO:0031333 [negative regulation of protein complex assembly] GO:0031424 [keratinization] GO:0031435 [mitogen-activated protein kinase kinase kinase binding] GO:0031647 [regulation of protein stability] GO:0031996 [thioesterase binding] GO:0032467 [positive regulation of cytokinesis] GO:0032488 [Cdc42 protein signal transduction] GO:0033138 [positive regulation of peptidyl-serine phosphorylation] GO:0034191 [apolipoprotein A-I receptor binding] GO:0034332 [adherens junction organization] GO:0034613 [cellular protein localization] GO:0035088 [establishment or maintenance of apical/basal cell polarity] GO:0035264 [multicellular organism growth] GO:0036336 [dendritic cell migration] GO:0036464 [cytoplasmic ribonucleoprotein granule] GO:0038096 [Fc-gamma receptor signaling pathway involved in phagocytosis] GO:0039694 [viral RNA genome replication] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042176 [regulation of protein catabolic process] GO:0042802 [identical protein binding] GO:0042995 [cell projection] GO:0043005 [neuron projection] GO:0043025 [neuronal cell body] GO:0043085 [positive regulation of catalytic activity] GO:0043197 [dendritic spine] GO:0043209 [myelin sheath] GO:0043410 [positive regulation of MAPK cascade] GO:0043497 [regulation of protein heterodimerization activity] GO:0043525 [positive regulation of neuron apoptotic process] GO:0043552 [positive regulation of phosphatidylinositol 3-kinase activity] GO:0045177 [apical part of cell] GO:0045740 [positive regulation of DNA replication] GO:0045859 [regulation of protein kinase activity] GO:0046330 [positive regulation of JNK cascade] GO:0046847 [filopodium assembly] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048013 [ephrin receptor signaling pathway] GO:0048664 [neuron fate determination] GO:0048730 [epidermis morphogenesis] GO:0051017 [actin filament bundle assembly] GO:0051022 [Rho GDP-dissociation inhibitor binding] GO:0051056 [regulation of small GTPase mediated signal transduction] GO:0051149 [positive regulation of muscle cell differentiation] GO:0051233 [spindle midzone] GO:0051246 [regulation of protein metabolic process] GO:0051301 [cell division] GO:0051489 [regulation of filopodium assembly] GO:0051491 [positive regulation of filopodium assembly] GO:0051647 [nucleus localization] GO:0051683 [establishment of Golgi localization] GO:0051835 [positive regulation of synapse structural plasticity] GO:0051988 [regulation of attachment of spindle microtubules to kinetochore] GO:0060047 [heart contraction] GO:0060070 [canonical Wnt signaling pathway] GO:0060071 [Wnt signaling pathway, planar cell polarity pathway] GO:0060501 [positive regulation of epithelial cell proliferation involved in lung morphogenesis] GO:0060661 [submandibular salivary gland formation] GO:0060684 [epithelial-mesenchymal cell signaling] GO:0060789 [hair follicle placode formation] GO:0060997 [dendritic spine morphogenesis] GO:0061630 [ubiquitin protein ligase activity] GO:0070062 [extracellular exosome] GO:0071338 [positive regulation of hair follicle cell proliferation] GO:0071944 [cell periphery] GO:0072384 [organelle transport along microtubule] GO:0072686 [mitotic spindle] GO:0090135 [actin filament branching] GO:0090136 [epithelial cell-cell adhesion] GO:0090316 [positive regulation of intracellular protein transport] GO:0099563 [modification of synaptic structure] GO:1900026 [positive regulation of substrate adhesion-dependent cell spreading]