Combining resources of high quality samples and affinity reagents to advance our understanding of human biology has driven the development of novel assay procedures and technologies.

Combining resources of high quality samples and affinity reagents to advance our understanding of human biology has driven the development of novel assay procedures and technologies. With the aim to establish a workflow for multiplexed analysis of human body fluids, alignment and optimisation of the different assay steps, used infrastructure and instruments is needed in order to provide the foundation for generating large sets of data.

On May 4th, Kimi Drobin defended her licentiate thesis entitled "Antibody-based bead arrays for high-throughput protein profiling in human plasma and serum" in which she presented her contribution to establishing the method for protein profiling in body fluids. Kimi has been a founding member of Plasma Profiling group and started her work at AlbaNova University Centre at the School of Biotechnology, KTH Royal Institute of Technology. In 2010, she and the team moved to SciLifeLab to build up the foundation of assays offered by the national facility.

Kimi has been involved in establishing the method (Drobin et al.; Häggmark et al.) for a variety of large-scaled screening projects performing protein profiling in different disease areas. Among others, Kimi recently focused on studying inflammatory bowel diseases (under review) and osteoporosis (Qundos et al.). She has further to this contributed with generating data leading to findings related to drug induced liver injury (Mikus et al.), multiple sclerosis (Byström et al. 2014) as well as malignant melanoma (Byström et al. 2017). Besides her research skills, Kimi has provided the group with inspiring artworks illustrating experimental concepts and theories.

References:

Drobin K, et al., (2013) Highly multiplexed antibody suspension bead arrays for plasma protein profiling. doi: 10.1007/978-1-4614-7209-4_8. PubMed PMID: 23765623.

Häggmark A et al., (2012). Classification of protein profiles from antibody microarrays using heat and detergent treatment. doi: 10.1016/j.nbt.2011.10.005.

Qundos U, et al. (2016) Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients. Proteomics Clin Appl. doi: 10.1002/prca.201400167

Mikus M, et al., (2017) Elevated levels of circulating CDH5 and FABP1 in association with human drug-induced liver injury. doi: 10.1111/liv.13174.

Byström S, et al. (2014) Affinity proteomic profiling of plasma, cerebrospinal fluid, and brain tissue within multiple sclerosis. doi: 10.1021/pr500609e.

Byström S, et al. (2017) Affinity Proteomics Exploration of Melanoma Identifies Proteins in Serum with Associations to T-Stage and Recurrence. doi: 10.1016/j.tranon.2017.03.002.