The cell is a multi-scale structure with modular organization across at least four orders of magnitude. Two central approaches for mapping this structure — protein fluorescent imaging and protein biophysical association — each generate extensive datasets, but of distinct qualities and resolutions that are typically treated separately. Here we present a computational framework to build a unified hierarchical map of human cell architecture...Read more

Endothelial cell (EC) dysfunction is a well-established response to cardiovascular disease risk factors, such as smoking and obesity. Risk factor exposure can modify EC signaling and behavior, leading to arterial and venous disease development. The aim was to identify biomarker panels for the assessment of EC dysfunction, which could be useful for risk stratification or to monitor treatment response...Read more

A new version 21 of the open access Human Protein Atlas has been launched. A lot of new data and content have been added and the resource now includes 10 separate sections exploring the human proteins from different angels. All data has been updated on the approximately 15 million individual web pages...Read more

In a study led by researchers in the HPA and at Brunel University London, a novel method for automated annotation of immunohistochemistry images was developed for annotating cell type-specific protein expression of 8 different cell types in human testis. The work comprised 7848 images (corresponding to 2794 proteins) and the image classifier also provided a novel uncertainty metrics (called DeepHistoClass), for identification of manual annotation errors. The workflow can be implemented for other tissues or utilized in large-scale protein mapping efforts for sourcing high-quality data...Read more